COMBINATION OF STATISTICAL TECHNIQUES FOR SUBMERGED FERMENTATION FOR EXTRACELLULAR POLYSACCHARIDE AND BIOMASS OF &lt;I&gt;GANODERMA TSUGAE&lt;/I&gt;

Nukrob Nukrob

doi:10.3844/amjbsp.2013.38.46

Abstract

Biomass and extracellular polysaccharide of Ganoderma tsugae have various biological activity including anti-inflamatory activity, antioxidant activity and antitumor activity. However, the growth rate of G. tsugae in nature is very slow. Therefore, many studies have attempted to develop mass culture systems for G. tsugae using laboratory techniques. Many parameters of submerged fermentation for G. tsugae were studies to determine the optimization of process by combination of statistical techniques. Ten parameters from preliminary results and literature reviews (maltose, skim milk, KH2PO4+K2HPO4, MgSO4-7H2O, CaCO3, vitamin B5+B6, olive oil, ethanol, pH and shaking speed) were screened by Packett Berman design. The significant parameters were determined the optimal ranges by path of steepest ascent method. The optimal condition of process was performed by response surface method. Maltose, skim milk and pH are significant parameters for G. tsugae cultivation. The conditions of 31.031 g L-1 maltose, 14.055 g L-1 skim milk and an initial pH of 7.12 resulted in the maximum extracellular polysaccharide content of 415 mg L-1 and the same fermentation broth at an initial pH of 6.46 exhibited the most biomass at 15.776 g L-1. Finally, the optimal condition was compared with un-optimal condition which result indicates that the combination of statistical techniques enhance the productions of biomass and extracellular polysaccharide (13X and 1.5X of the control, respectively). Therefore, these strategies are useful for improvement of submerged fermentation of G. tsugae which it can apply in pharmaceutical industry.

Highlights

Song San Ling Chih or Ganoderma tsugae has been as a traditional medicine which it is used to promote of health and longevity in Asian countries (Lin et al, 2013)
The optimal condition was compared with un-optimal condition which result indicates that the combination of statistical techniques enhance the productions of biomass and extracellular polysaccharide (13X and 1.5X of the control, respectively)
Carbon source as maltose, nitrogen source as skim milk and vitamin B5+B6 were nutrients used during fermentation screening by Plackett Burman Design (PBD), whereas the other nutrients and fermenting conditions were set base on literature reviews

Summary

Introduction

Song San Ling Chih or Ganoderma tsugae has been as a traditional medicine which it is used to promote of health and longevity in Asian countries (Lin et al, 2013). The polysaccharides produced by G. tsugae have been studied and used for pharmaceutical purposes as treatment for various diseases (Hsu et al, 2009) and have been applied to add in the food and medicinal industries (Rusell and Paterson, 2006). The cultivation of G. tsugae for fruiting body takes a long period and high risk of contamination from nature. Technique of submerged fermentation was interested from biopharmaceutical industry, because it has short time cultivation, easy to control condition for contaminated protection and obtain best product in quality and quantity (Narkprasom et al, 2012). The optimization method for many parameters in fermentation process of G. tsugae has attracted significant interest from pharmaceutical industry.

Objectives

Methods

Results

Discussion

Conclusion