Combination of polymer-bound charged groups and affinity ligands for extraction of enzymes by partitioning in aqueous two-phase systems

Abstract

The partition of glucose-6-phosphate dehydrogenase in a water—dextran—polyethylene glycol two-phase system was influenced by the mutual addition of polymers carrying charged groups and affinity ligands. These polymers included DEAE-dextran, dextran sulphate, ligand—dextran, ligand—polyethylene glycol, ligand—Ficoll and ligand—DEAE-dextran, where the ligands used were the dyes Cibacron Blue F3G-A and Procion Yellow HE-3G. Some of these two-phase systems were also used for the counter-current distribution of yeast proteins, demonstrating the separation of several enzymes.