Combination of MS medium and Gandasil D on banana shoots growth in vitro

Abstract

Cavendish banana has a high economic for food export commodities. High export opportunities need to be balanced with increased productivity by selecting superior seedlings through in vitro seed propagation. The tissue fruitfulness depends on the media used. The media used substitution between MS media and Gandasil D leaf fertilizer with different concentrations. The researcher also used a completely randomized design (CRD) of one factor with five treatment combinations and six repetition. The treatment used is MS 100% as a control; 75% MS + 25% Gandasil D; 50% MS + 50% Gandasil D; 25% MS + 75% Gandasil D; 100% D. the variables observed include time of buds, number of shoots, plantlet height, time of leaves, number of leaves, time of roots, number of roots, and root length. The data analysis used is the physical analysis method based on the F test of 5% level. The results of the research showed that 100% Gandasil D and 25% MS + 75% Gandasil D treatment had the highest influence on the number of leaves, number of shoots, plant height, and number of roots. The mixture of MS media and leaf fertilizer can be used as an alternative medium for multiplication of Cavendish bananas.