Combination of Moringa oleifera leaf extract and demineralized freeze-dried bovine bone xenograft (DFDBBX) on woven bone area post tooth extraction

Abstract

Background: Tooth extraction is one of the most frequently performed procedures in dental practice. Resorption without bone regeneration can change the maxillary and mandibular bone structure, resulting lack of retention, stability, and comfort of the denture. Hence, adequate regeneration of alveolar bone is considered an important factor for successful dentures. Moringa oleifera is a plant rich in flavonoids, saponins, alkaloids and tannins. Purpose: This study aims to find out the effect of induction from combination of Moringa oleifera extract and demineralized freeze-dried bovine bone xenograft (DFDBBX) on woven bone area post tooth extraction procedure. Methods: Cavia cobaya was divided into eight groups, each group consists of seven. The lower incisors of Fifty-six Cavia cobaya were extracted. The first group (control group) was given polyethylene glycol, the second group with DFDBBX, the third group with Moringa leaf extract, and the fourth group with a combination of DFDBBX and Moringa leaf extract. Cavia cobaya were sacrificed on day 7 and 14. Histopathological examinations of woven bone area was observed under a 400x magnification light microscope. Kruskal-Wallis and Mann-Whitney tests were performed to analyze the data. Results: There was a significant difference in woven bone area between the groups (P<0.05). The highest mean of woven bone area was found in the combination group on both day 7 and 14. Conclusion: The combination of Moringa leaf extract and DFDBBX could effectively formed new woven bone during the alveolar bone regeneration.