Abstract
Egg yolk granule phosvitin (45 kDa) is a phosphoprotein known for its emulsifying properties. Recently, high hydrostatic pressure (HHP) treatment of granule induced the transfer of phosvitin to the soluble plasma fraction. This project evaluated the performance of the ultrafiltration (UF) used to concentrate phosvitin from the plasma fraction to produce a natural emulsifier. Phosvitin was characterized in plasma from a pressure-treated granule (1.73 ± 0.07% w/w) and in its UF retentate (26.00 ± 4.12% w/w). The emulsifying properties of both retentates were evaluated. The emulsion prepared with phosvitin-enriched retentate was more resistant to flocculation and creaming. Confocal laser scanning microscopy showed a network of aggregated protein similar to a gel, which encapsulated oil droplets in emulsions made with UF-retentate of plasma from pressure-treated granule. However, although sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that β-phosvitin is recovered in the cream, it is difficult to attribute the improved emulsifying properties of the UF-retentate of plasma from pressure-treated granules only to phosvitin.
Highlights
Phosvitin represents 4% w/w of the dry matter and 11% w/w of the protein in egg yolk [1]
Green and Nile Red to observe proteins and fat, respectively. These results suggest that the combination of high hydrostatic pressure (HHP) and UF might be interesting for industrial
Thesefor results suggest that combination ofin and UFThe might be interesting for industrial purposes the production of athe fraction enriched phosvitin
Summary
Phosvitin represents 4% w/w of the dry matter and 11% w/w of the protein in egg yolk [1]. Present in the granule fraction as non-soluble high-density lipoprotein (HDL)-phosvitin complexes linked by phosphocalcic bonds, phosvitin is generated by the cleavage of vitellogenin [2] and is found in three isoforms: α1, α2 and β-phosvitin [3,4]. These isoforms are composed of three to four subunits of 35 to 40 kDa for α-phosvitins, and four to five subunits of 45 kDa for β-phosvitin [3]. Phosvitin is recognized for its antimicrobial activity [13], its action against
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