COMBINATION OF GLICLAZIDE DRUG AND LUPIN SEEDS POWDER ALLEVIATE HYPERGLYCEMIA ON INDUCED-DIABETIC RATS RECEIVING HIGH-FAT HIGH FRUCTOSE/SUCROSE DIET

Abstract

Diabetes mellitus is one of the most prevalent metabolic diseases in the world. Hyperglycemia and lipoprotein abnormalities are the characteristic clinical finding of DM. The most common legume food used in traditional medicine for the treatment of diabetes is lupin seeds powder. The aim of current research is to analyze the therapeutic effects of the combination of both hypoglycemic drug (Gliclazide) and lupin seeds powder on some biochemical parameters (serum blood glucose, serum insulin, glyclated hemoglobin and total lipid profile) and also on mRNA expression level of Glucokinase, Phosphoenolpyruvate carboxykinase (PEPCK), Insulin and Peroxisome Proliferator-Activated Receptor-Gamma (PPAR-γ) genes using relative quantitative PCR. In this study, thirty zucker male rats were divided into two groups: normal control group (six rats) and the other group expose to induce type2 DM by high-fat high fructose/sucrose diet. The diabetic groups were sub classified into 4groups (6 rats in each group), one group diabetic control and the rest was treated with Gliclazide (10mg/kg),combination of Gliclazide(10mg/kg) and lupin seeds powder (1gm/kg) and lupin seeds powder (1gm/kg) only. Combination between Gliclazide (10mg/kg) and lupin seeds powder (1gm/kg) daily for 4 weeks resulted in significant decrease in blood glucose level, glyclated hemoglobin and dyslipidemia (p<0.05), also results in significant increase (P<0.05) in high density lipoprotein-cholesterol(HDL-c) (90.66±3.8), insulin level(4.9±0.058). Also the combination of Gliclazide (10mg/kg) and lupin seeds powder (1gm/kg) tends to return biochemical parameters, lipid profile (triacylglycerol 77±3.05, total cholesterol 132±2) and the transcription of mRNA metabolic genes to normal levels ( Glucokinase 1.4±0.1, insulin 1.48±0.25, PEPCK 0.49±0.25, PPAR-γ 3.1±0.2 ) more than the treatment with Gliclazide drug only, in type 2 diabetic rats. It is concluded that, the combination of Gliclazide hypoglycemic drug and lupin seeds powder has the most hypoglycemic effect when compared to other groups.