Combination of Different Antagonistic Bacteria to Control of Potato Blackleg Disease Caused by Pectobacterium atrosepticum under Greenhouse and Field Conditions

Hoda H El-Hendawy,Kamal A M Abo-Elyousr

doi:10.33687/phytopath.005.01.1647

Hoda H El-Hendawy, Kamal A M Abo-Elyousr

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https://doi.org/10.33687/phytopath.005.01.1647

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Abstract

Three different antagonistic bacterial isolates, Pseudomonas fluoresces (Pf2), Bacillus subtilis(Bs3) and Rahnella acquatilis (Ra39) restricted the growth of Pectobacterium atrosepticum, the causal agent of black leg disease of potato, in vitro. Under greenhouse and field conditions, potato plants pre-treated with the three antagonistic bacterial isolates, individually or in combination, showed reduced disease severity relative to non-treated control plants. All isolates produced siderophores in different degrees but did not produce indole acetic acid (IAA) or hydrogen cyanide (HCN).The obtained results indicated that combination of Pseudomonas fluoresces (Pf2), Bacillus subtilis (Bs3) and Rahnella acquatilis (Ra39) is beneficial in controlling black leg disease of potato caused by pectobacterium atrosepticum.

Highlights

International Journal of PhytopathologyISSN: 2305-106X (Online), 2306-1650 (Print) http://www.escijournals.net/phytopathology UNDER GREENHOUSE AND FIELD CONDITIONS aHoda H. El-Hendawy, bKamal A. M. Abo-Elyousr* a Botany and Microbiology Department, Faculty of Science, Helwan University, Ain Helwan, Cairo, Egypt. bPlant Pathology Dept. Faculty of Agriculture, Assiut University - 71526, Assiut, Egypt
Potato (Solanum tuberosum, L.) is an important food source, it is theworld,s fourth main food crop after rice (Oryza sativa, L.), maize (Zea mays, L.) and wheat (Triticum aestivum, L.) in cultivated area and total production (Douches et al, 1996)
In an attempt to find biological control agents against Pectobacterium atrosepticum, three bacterial strains, P. fluorescnes (Pf2), B. subtilis (Bs3) and Rahnella aquatilis (Ra39) were screened for their in vitro antibiosis towards P. atrosepticum and proved to be able to restrict the growth of the bacterial pathogen

Summary

International Journal of Phytopathology

ISSN: 2305-106X (Online), 2306-1650 (Print) http://www.escijournals.net/phytopathology UNDER GREENHOUSE AND FIELD CONDITIONS aHoda H. El-Hendawy, bKamal A. M. Abo-Elyousr* a Botany and Microbiology Department, Faculty of Science, Helwan University, Ain Helwan, Cairo, Egypt. bPlant Pathology Dept. Faculty of Agriculture, Assiut University - 71526, Assiut, Egypt.

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