Abstract
Tolclofos-methyl (TM) is an organophosphorus fungicide and widely utilized to control soil-borne diseases. However, toxic effects of TM on terrestrial invertebrates are still unknown. Here we measured the bioaccumulation of TM in earthworms (Eisenia fetida) to assess its environmental bioavailability. Mortality, weight change, and oxidative damage of earthworms were determined to investigate the toxicological bioavailability of TM. ROS, SOD and MDA in highest concentration treatment group significantly increased compared to the control group, suggesting that hazardous effects of TM to earthworms were caused by the oxidative stress. To further examine its toxicological bioavailability, cytotoxicity test was carried out by using extracted earthworm coelomocytes. The biomarkers, e.g., intracellular ROS, extracellular LDH, and cell viability showed correlation with TM in the culture media, demonstrating that cytotoxicity test could be employed to reflect the toxicological bioavailability of pollutants to earthworms or other organisms.
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