Abstract
We investigated the combination effect of ozone and heat treatments in apple juice for the inactivation of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes. Apple juices inoculated with the three pathogens were treated with gaseous ozone and heat simultaneously for up to 1min. Gaseous ozone treatment was progressed at a flow rate of 3.0l/min with a concentration of 2.0–3.0g/m3 and heat treatment was performed at temperatures of 25, 45, 50, and 55°C. Populations of surviving pathogens decreased in all samples as treatment temperature increased from 25 to 55°C. Heat treatment alone (25, 45, 50 and 55°C) resulted in 0.20, 0.37, 2.16 and 2.54logCFU/ml reductions of E. coli O157:H7, respectively, in apple juice. Combination treatment of ozone and heat for 1min reduced this pathogen by 1.50 and 1.60logCFU/ml, respectively, at 25 and 45°C, and below the detection limit (1logCFU/ml) at 50 and 55°C. We found a synergistic effect in the inactivation of pathogens in apple juice treated with ozone and heated at 50°C. The reduction trend of S. Typhimurium and L. monocytogenes in apple juice was similar to that of E. coli O157:H7. There were no significant changes of Hunter color values when apple juices were treated with heat only and the combination of ozone and heat. Residual ozone was measured following ozone treatment. In all ozone treated samples, the concentration of residual ozone was reduced to under acceptable levels (<0.4mg/l). In conclusion, the combination treatment of ozone and heat was significantly effective in the inactivation of foodborne pathogens while maintaining acceptable apple juice quality.
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