Abstract
BackgroundPeriodontitis is a chronic inflammatory disease characterized by progressive damage on the structure of tooth-supporting tissues. The aim of the study is determining the combination photodynamic effect of diode laser 405 nm treatments and the administration of doxycycline 0.1% within 1, 3, 5, and 7 days on a Wistar rat model of periodontitis.MethodsSamples were induced with Porphyromonas gingivalis ATCC33277 to allow periodontitis development and were treated with combination of doxycycline and laser diode, then statistical analysis was carried out (One-Way ANOVA test and the post-hoc Duncan test; Kruskal–Wallis test and Mann–Whitney follow-up test for non-parametric data). Samples were divided into five groups, laser exposure used was 405-nm diode laser with energy density of 8 J/cm2. The expression level of histomorphometric was calculated by measuring the number of macrophages, lymphocytes, fibroblasts and the distance between the CEJ-AV.ResultsThe results showed that the combination treatment of doxycycline and laser exposure yielded immunomodulatory effects. The expression level of fibroblast and the distance between CEJ-AV bone showed that the combination of doxycycline and laser therapy exerted healing effect in rat models of periodontitis on day 5 and 7.ConclusionThe combination of doxycycline 0.1% and diode laser therapy provides a healing effect in rats models of periodontitis.
Highlights
Periodontitis is a chronic inflammatory disease characterized by progressive damage on the structure of tooth-supporting tissues
The wavelengths are in accordance with the absorption spectrum range of both Porphyromonas gingivalis bacteria and doxycycline that were used as exogenous photosensitizers in this study
The results showed that the combination treatment of doxycycline 0.1% and laser exposure yielded immunomodulatory effects
Summary
Periodontitis is a chronic inflammatory disease characterized by progressive damage on the structure of tooth-supporting tissues. Periodontitis is chronic inflammatory disease characterized by progressive damage on the tooth-supporting tissue structure [1]. Treatment and prevention methods that have been used so far only aim to eliminate. The mechanical process, either by brushing teeth or the Scale Root Planning (SRP) method, are not effective in removing plaque from the teeth because the probe could not reach a tooth area of > 5 mm [8]. The use of antibiotics is an effective method for bacterial colonies removal, as stated by the World Health Organization in 2014 [9]. One alternative treatment modality that would help overcome this problem is photodynamic therapy
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