Abstract
Solanum tuberosum cv. Pentland Dell expresses single gene-mediated hypersensitive resistance (HR) to potato virus Y (strain group PVY O), A (PVA 1) and X (PVX 3) and is resistant to cucumber mosaic virus (Fny-CMV). S. brevidens is resistant to these viruses, too, but resistance is functional through slow virus cell-to-cell movement. These two virus resistance mechanisms were combined by symmetric somatic hybridization. Six hybrid plants were regenerated from a single fusion callus and tested for virus resistance by graft-inoculation. Titers of PVY and PVA in the hybrids were higher than in S. brevidens. Only limited necrosis developed following infection with PVY and chlorotic lesions developed following infection with PVA in the hybrids, whereas top necrosis and necrotic lesions, respectively, developed in P. Dell. No virus or symptoms were observed in one hybrid inoculated with PVX and three hybrids inoculated with CMV. Other hybrids developed lethal necrosis following infection with PVX, and stunting, necrosis and yellow mosaic symptoms following infection with CMV, which was similar to P. Dell. S. brevidens contained moderate titers of PVX and CMV. It developed no symptoms following infection with PVX and only mild mosaic symptoms following infection with CMV. Altered resistance expression in the hybrids may be attributable to simultaneous expression of the two resistance mechanisms, interference in the expression of either resistance mechanism caused by the genes of the heterologous genome, and/or somaclonal variation.
Published Version
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