Abstract
A single human red blood cell was optically stretched along two counter-propagating fiber-optic Bessel-like beams in an integrated lab-on-a-chip structure. The beam enabled highly localized stretching of RBC, and it induced a nonlinear mechanical deformation to finally reach an irreversible columnar shape that has not been reported. We characterized and systematically quantified this optically induced mechanical deformation by the geometrical aspect ratio of stretched RBC and the irreversible stretching time. The proposed RBC mechanism can realize a versatile and compact opto-mechanical platform for optical diagnosis of biological substances in the single cell level.
Highlights
Since Ashkin experimentally demonstrated optical trapping and transportation of dielectric particles, optical manipulation of microscopic living objects has been one of the prominent research fields in biomedical photonics [1]
We demonstrated a unique columnar deformation of red blood cell (RBC) using dual fiber optic Bessel-like beams on a lab-on-a-chip system
The aspect ratio R of the stretched RBC revealed a clear distinction between the reversible stretching region (R~1.5) and the irreversible stretching region (R > 4.2)
Summary
Since Ashkin experimentally demonstrated optical trapping and transportation of dielectric particles, optical manipulation of microscopic living objects has been one of the prominent research fields in biomedical photonics [1]. Guck et al [5,6,7] further developed the single RBC stretching using two counter-propagating Gaussian beams without attaching dielectric beads, and estimated the stretching stress on RBC as a function of the laser power. Their Gaussian beam dimension was larger than that of the RBC and only the spatially averaged elastic properties of RBC were attainable, and the localized deformation of RBC beyond the elastic regime has not been discussed in detail. The proposed configuration enabled systematic investigation on the nonlinear viscoelastic behavior of RBC with a low optical power, which can be further used as a bio-photonic signature to assess the status of RBC healthiness
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