Abstract
The separation of DDT, certain of its homologs and analogs (DDE, DDD, DBP, Kelthane, and FW-152) which are of interest in studies of detoxification of DDT, and some derivatives of DDT (Cl-DDT, Ac-DDT) was achieved by column chromatography of the compounds partitioned between 2,2,4-trimethylpentane (moving phase) and 3-methoxypropionitrile (stationary phase) on cellulose powder. Measurement of the absorbancies of the effluent fractions at 235 and 268 mμ served as the primary quantitative and qualitative assay. A colorimetric procedure, modifying earlier applications of the Fujiwara reaction, was developed to measure Kelthane and Ac-DDT.
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