Abstract
Injecting too much sample onto a chromatographic system will eventually overload the column. Analytical chemists are concerned about column overloading because it reduces column efficiency, and, as a result, the resolution is sacrificed. Surprisingly, there is little data in the literature about the overloading of the porous layer open tubular (PLOT) columns. This month’s article looks at the basic types of column overload, volume and mass, and defines the variables that most affect the sample loading capacity in PLOT column chromatography.
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