Column bioreactor of immobilized Stropharia sp. ITCC 8422 on natural biomass support of L. cylindrica for biodegradation of anthraquinone violet R

Abstract

The column bioreactor of fungal biomass Stropharia sp. ITCC - 8422 was immobilized on natural biomass support of L. cylindrica and effectively decolorized anthraquinone violet R (AQVR). The designed column bioreactor successfully decolorized >80% AQVR up to third cycle whereas >55% AQVR decolorization was observed up to 7 cycles. Regenerated column reactor had improved efficiency and was used for multiple cycles. No significant adsorption on immobilized fungal and natural biomass was observed. The crude yellow laccase (YL) decolorized >80% AQVR (100 ppm) at pH 9 and 40 °C after 10 min. Untreated and treated AQVR were examined using spectral studies and the degraded products were confirmed using Q-TOF LCMS and pathway was proposed. AQVR was broken down to low molecular weight products i.e., m/z = 146.0607 and 130.064. The degraded products were less toxic and confirmed using phyto-toxicity test.