Abstract
Zidovudine (ZDV) is an antiviral drug against HIV that was approved by the FDA on March, 1987. It is a reverse transcriptase inhibitor. This type of drug stops the reproduction of DNA and decreases the amount of the virus in the patients' blood. Due to the ability of forming various molecular bonds, silver nanoparticles (AgNPs) are widely used for the detection of large range of agents, including drugs. In this study, we synthesized AgNP-modified β-cyclodextrin (β-CD) using green synthesis for the sensitive and selective zidovudine (ZDV) determination. Characterization of nanoparticles was done using different methods including infrared (IR) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), and also, X-ray diffraction (XRD) patterns. The AgNP λ-max peak was at approximately 405 nm. In the presence of ZDV, yellow solution was turned to red color, and surface plasmon absorption band was dramatically centered at 560 nm. ZDV was determined in the range of 50–500 μM, and the detection limit value was obtained as 42 μM. The sensor was used to determine ZDV in tablets with good recovery.
Highlights
Zidovudine (ZDV), known as 3-azido-3-deoxythymidine, is a chemical component which can be phosphorylated to ZDV triphosphate in the body. e research studies indicated that phosphorylate form could prevent HIV replication with inhibition of the reverse transcriptase as a key enzyme
3.1. β-CD-AgNP Characterization. e synthesized AgNPs were fully characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), and UV-Vis spectra
TEM analysis further confirmed the formation of silver nanoparticles
Summary
Zidovudine (ZDV), known as 3-azido-3-deoxythymidine, is a chemical component which can be phosphorylated to ZDV triphosphate in the body. e research studies indicated that phosphorylate form could prevent HIV replication with inhibition of the reverse transcriptase as a key enzyme. Because of excellent plasmon absorption properties of novel metal nanoparticles (NPs), especially gold nanoparticles (AuNPs) [5] and AgNPs [6], a variety of colorimetric sensors have been developed [7, 8] It is a sensor by which the detection of various analytes could be recognized with naked eyes [8, 9]. Due to unique properties including antibacterial and thermal activity, surface plasmon resonance (SPR), and catalytic and electronic properties, AgNPs have been applied remarkably in various fields [12, 14,15,16,17,18] Some other materials such as chitosan [19] and cyclodextrin (CD) [20] have been used to attach silver. Color of AgNP solution has been changed by adding different ZDV concentrations
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
