Colorimetric sensing of using N′-(pyrene-1-ylmethylene) benzene-1,2-diamine/triiodide ions conjugate in human serum

Abstract

As an important neurotransmitter, dopamine has been playing a vital role in central nervous system and its variation of levels has leads to plenty of neurological and psychiatric disorders. Among good number of colorimetric dopamine biosensors, our group has designed more sensitive colorimetric sensor and reported as first organic chromophore based sensor for dopamine. The developed sensor is a combination of simple conjugate namely N′-(pyrene-1-ylmethylene) benzene-1,2-diamine (PMBD)/triiodide ions. Under optimized conditions, the sensor has shown wide range of detection towards dopamine from 5 to 475 pM range and the LOD was calculated as 0.02 pM. Interestingly, the sensing range of our sensor falls in pM level, it is more useful in clinical applications because the dopamine concentration may varies from pM to nM level in human serum at different parts of the human body. The real time analysis of the developed sensor has been tested in human serum samples collected from different persons. This physiological detection ranges captivates by the clinical technicians for the diagnosis of dopamine in serum for various brain related issues.