Abstract
Monitoring of the organophosphorus pesticides dichlorvos at very low levels has been achieved with liposome-based nanobiosensors. The enzyme acetylcholinesterase was effectively stabilized within the internal nanoenvironment of the liposomes. Within the liposomes, the pH sensitive fluorescent indicator pyranine was also immobilized for the optical transduction of the enzymatic activity. Increasing amounts of pesticides lead to the decrease of the enzymatic activity for the hydrolysis of the acetylcholine and thus to a decrease in the fluorescent signal of the pH indicator. The decrease of the liposome biosensors signal is relative to the concentration of dichlorvos down to 10-12 M levels. Also a colorimetric screening device for pesticide analysis has been evaluated.
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