Abstract
Abstract A colorimetric method for determining thymol and eugenol in volatile oils is presented. Thymol and eugenol are reacted with sodium cobaltinitrite in aqueous acetic acid. The yellow color produced is measured at 330 and 370 nm in chloroform, and at 396 and 450 nm in sodium hydroxide solution for thymol and eugenol, respectively. The method is applied to the assay of thyme oil (thymol type) and clove oil. The results obtained are reasonably reproducible with a coefficient of variation less than 2%.
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