Abstract
The colorimetric methods in common use for determining and adjusting the reaction of bacteriologic mediums and other fluids involve the use of standard solutions of known hydrogen ion concentration. A variety of such solutions has been described, and they are readily prepared by any one having a moderate chemical equipment and ability. Just now, however, many men with comparatively little chemical training are confronted with problems of this nature, and the method here described has therefore been devised to accomplish a fairly accurate determination of hydrogen ion concentration without the necessity of preparing standard solutions or of depending on those prepared by others. We are here concerned only with reactions lying between 7.0 and 8.0, and only phenolsulphone-phthalein has been used as an indicator. Other indicators and other ranges of acidity will be investigated later. In this method use is made of the principle of superimposing the two extreme colors
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