Abstract
By diazotization coupling reaction is one of the most frequently employed technique in pharmaceutical analysis. It involves measuring the amount of ultraviolet or visible radiation absorbed by a substance in solution as a method for estimation drug of Phenylephrine hydrochloride by reliant on azo-coupling reaction between (PHE)HCl and Clonazepam as a reagent. To reach to the aim of obtaining the colored product with a maximum absorbance 466nm. The linearity range for Phenylephrine hydrochloride was (0.5_9μg\\ml) while the detections limit 0.006 and quantification limit 0.02μg\\ml The Molar Absorptivity 2.5457 ×104L/mol.cm with Sandal’s sensitivity 0.01μg\\cm2. Eventually these methods were successfully completed to determination of PHE.HCl in pure and commercial form.
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