Abstract
THE sugar 3,6-anhydrogalactose is a major constituent of the agarose fraction of agar, of kappa carrageenin, and of some other polysaccharides from marine algae1. This sugar can be determined spectrophotometrically as 5-hy-droxymethyl-2-furaldehyde2 or by an adaptation of the rosorcinol test for ketoses3. The rate of degradation of 3,6-anhydrogalactose to 5-hydroxymethyl-2-furaldehyde suggests that any method used for the colorimetric determination of fructose should be applicable to 3,6-anhydrogalactose. Steinitz4 compared six methods for the determination of fructose in body fluids, and favoured the method of Heyrovsky5, who used β-indolylacetic acid as the colour reagent. The present communication describes the use of this reagent for the determination of 3,6-anhydrogalactose.
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