Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles.

Abstract

We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs). In this study, ssDNA aptamer of S. typhimurium could combine with CTAB to form the supramolecular ssDNA-CTAB composite, so the ssDNA aptamer was applied to control the concentration of CTAB. In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host-guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change. The ratio of absorption at 650 and 520nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102-1 × 107CFU/mL) with a low limit of detection (LOD) of 13CFU/mL. In addition, this optical sensor has good selectivity and practicability. In milk samples, the recovery was 93.55-111.32%, which suggested its potential application in real samples.