Abstract
A facile colorimetric assay for the detection of lipopolysaccharides (LPSs) was proposed based on a LPS-binding peptide and AuNPs.
Highlights
Lipopolysaccharides (LPSs), known as bacterial endotoxins, are the major structural constituent of the outer membrane of Gram-negative bacteria, contributing greatly to the integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack.[1,2] LPSs are comprised of a variable polysaccharide and a conserved glucosamine-based phospholipid called lipid A, which anchors the LPS into the membrane.[3,4] The release of the LPS from bacteria can elicit host monocytes and macrophages to secrete a broad range of in ammatory cytokines, such as tumor necrosis factor-a, interleukin-1, and interleukin-8.5,6 Massive secretion of these cytokines can lead to diarrhea, septic shock and even death
Innovative attempts have shown great promise with their individual limit of detection ranging from the micromolar to the low nanomolar level, the construction of the sensors usually involves very sophisticated organic synthesis
The aggregation of AuNPs induced by different concentrations of the LPS-binding peptide (LBP) were investigated, and the results are shown in Fig. S4.† Upon the addition of the LBP, the absorbance at 518 nm (A518) decreased, while that at 586 nm (A586) increased (Fig. S4A†)
Summary
Colorimetric detection of lipopolysaccharides based on a lipopolysaccharide-binding peptide and AuNPs† Cite this: Anal. A novel label-free colorimetric assay for the detection of lipopolysaccharides (LPSs) was developed based on a LPS-binding peptide (LBP) and unmodified gold nanoparticles (AuNPs). The proposed assay is capable of sensitively and selectively measuring LPSs in the range of 10–1000 nM with a detection limit of 2.0 nM
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