Colorimetric Detection of Glucose in Biological Fluids Using Toner-Based Microzone Plates

Paula B M Silva,Karoliny A Oliveira,Wendell K T Coltro

doi:10.5935/0103-5053.20160164

Paula B M Silva, Karoliny A Oliveira + Show 1 more

Open Access

https://doi.org/10.5935/0103-5053.20160164

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Abstract

This report describes the use of toner-based microzone plates for quantitative determination of glucose in artificial urine and human serum samples using colorimetric detection. The proposed approach has exhibited a linear response for glucose concentration levels from 0 to 10 mmol L-1 with determination coefficient (R2) of 0.996. The achieved values for sensitivity and limit of detection were 5.1 AU (mmol L-1)-1 and 0.6 mmol L-1, respectively. The glucose analysis in artificial serum samples revealed error lower than 5% in comparison with certified values provided by the supplier. Lastly, the proposed approach has also provided suitable accuracy (92-105%) for glucose measurements in artificial urine samples.

Highlights

In the last years, the miniaturization of analytical systems has received considerable attention due to their attractive advantages including low reagent and sample consumption, portability, minimal waste generation, short time analysis, and high-throughput capability.[1,2,3,4,5,6,7] Standard photolithography is one of the main microfabrication technologies allowing the fabrication of microfluidic channels on different platforms including glass, silicon and quartz
Due to the advantages associated with the fabrication simplicity and low cost per device, some authors have extended the use of laser printing technology (LPT) to produce toner-based devices for clinical assays involving the use of portable equipment like cell phone camera, scanner, digital camera and handheld optical microscope for colorimetric measurements
We describe the use of tonerbased microzone plates for glucose analysis in biological fluids using colorimetric measurements

Summary

Introduction

The miniaturization of analytical systems has received considerable attention due to their attractive advantages including low reagent and sample consumption, portability, minimal waste generation, short time analysis, and high-throughput capability.[1,2,3,4,5,6,7] Standard photolithography is one of the main microfabrication technologies allowing the fabrication of microfluidic channels on different platforms including glass, silicon and quartz. Laser printing technology (LPT) has been used to produce microfluidic toner-based analytical devices (μTADs) at very low cost. Due to the advantages associated with the fabrication simplicity and low cost per device, some authors have extended the use of LPT to produce toner-based devices for clinical assays involving the use of portable equipment like cell phone camera, scanner, digital camera and handheld optical microscope for colorimetric measurements.

Results

Conclusion