Abstract
According to the color development reaction between starch/iodine reagent and products which obtained by fumigating melamine with chlorine, a method for determination of melamine in milk was put forward. The conditions of method were researched. The best conditions are as follows. Volume ratio of milk and extraction agent is 1:1. 5, dissolved with ultrasonic washer for 5 minutes, centrifugal separation 4000r/min for 5 minutes, extracted by C008 cation exchange resin column. The results are as follows: detection limit is 10mg/L, the linear range is 25-250 mg/L, related coefficient r is 0.994, coefficient of recovery is 94%-110%, and relatively standard deviation is 0.9%. This method is easy handling, cheap equipment, good practicability and recovery, can be used as low cost detection method.
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