Colonization of carnation stems by a nonpathogenic isolate of Fusarium oxysporum and its effect on Fusarium oxysporum f.sp. dianthi

Abstract

A nonpathogenic isolate of Fusarium oxysporum, 618-12, added to soil prior to the pathogen, suppressed fusarium wilt (F. o. f.sp. dianthi race 2) in a susceptible cultivar of carnation by 80% compared with the treatment with the pathogen only. The possibility of systemically induced resistance by the nonpathogenic isolate was assessed by inoculating antagonist and pathogen at different locations (stem versus soil, soil versus stem, and in a split-root system). No significant disease suppression was found with any of these spatially separated inoculations. However, inoculation of antagonist and pathogen at the same location within the stem (i.e., mixed stem inoculation) resulted in significant and reproducible disease reductions compared with stem inoculation with the pathogen alone. This reduction was found for different inoculum densities and different cultivars. Several other nonpathogenic Fusarium isolates could also reduce wilt symptoms in the susceptible carnation cultivar after mixed stem inoculation with the pathogen. This disease-suppressive effect after mixed stem inoculations may be caused by locally induced resistance or competition between isolates within the stem. Plants showed vascular browning around the inoculation point following inoculation with nonpathogenic isolates. Disease suppression, as well as vascular browning, were absent when dead conidia of the isolate 618-12 were used. After its addition to soil, the isolate was recovered from 44–78% of carnation stems. Spread of the nonpathogenic isolate within the stem occurred only in the first 4 days after stem inoculation, and it remained confined to limited distances from the inoculation point between 4 and 59 days after inoculation. The pathogenic isolate could be isolated at increasingly greater distances from the inoculation point during this period. These data suggest that the nonpathogenic isolate is not actively spread through the plant by growth of the fungus. Keywords: biological control, competition, induced resistance, wilt.