Abstract
Neurons in the septum (SEP) and vertical limb of the diagonal band (vDB) of 6-day-old rats were dissociated and cultured for 3 days. Cells with high affinity uptake mechanism for γ-aminobutyric acid (GABA) were identified autoradiographically using 16 nM [ 3H]GABA. Cells that expressed nerve growth factor (NGF) receptor immunoreactivity were identified immunocytochemically using MC192, a monoclonal antibody against NGF receptor. Double labeling experiments combining [ 3H]GABA uptake with immunostaining for NGF receptor showed that 46% of the NGF receptor-immunoreactive cells accumulated [ 3H]GABA. The result was discussed in relation to a possible involvement of the developing septal GABAergic neurons in NGF-induced neuronal survival.
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