Co-localisation of neuropeptides with kisspeptin in preoptic/hypothalamic neurons of the mouse brain

Abstract

Event Abstract Back to Event Co-localisation of neuropeptides with kisspeptin in preoptic/hypothalamic neurons of the mouse brain Barbara Vida1, Levente Deli1, Theodosis Kalamatianos2, Erik Hrabovszky1, Alain Caraty3, Coen W. Clive2, Zsolt Liposits1 and Imre Kalló1* 1 Hungarian Academy of Sciences, Hungary 2 King’s College London, United Kingdom 3 NRA/CNRS/University of Tours/Haras Nationaux, France Besides the classical neurotransmitters, neuropeptides also play significant roles in the regulation of gonadotropin releasing hormone (GnRH) secretion. Galanin and neurokinin B (NKB) have a modulatory function on GnRH secretion. In contrast, kisspeptin (KP) initiates a long-lasting activation of GnRH neurons. KP neurons in the rostral periventricular area of the third ventricle (RP3V) have been shown recently to convey estrogens’ positive feed-back action on GnRH secretion, whereas kisspeptin neurons located in the arcuate nucleus (Arc) have been implicated in the negative feed-back effects of estrogen. The aim of this study was to determine, whether galanin or NKB are co-expressed with kisspeptin in the mouse RP3V and/or Arc or alternatively, they are synthesized in different subsets of cells. Dual-label immunohistochemistry for KP and preproneurokinin B or galanin was carried out on sections from colchicine-treated female mice representing the full rostrocaudal extent of either the RP3V or the Arc. Maximum kisspeptin expression was detected in the RP3V of sections originating from ovariectomised (OVX) and estrogen-treated females, whereas the Arc showed the highest levels in sections derived from OVX females. Confocal microscopic analysis revealed that almost all KP neurons in the Arc, but none in the RP3V express NKB. In contrast, galanin immunoreactivity was observed in nearly every KP neurons in the RP3V, whereas in the Arc galanin immunoreactivity was detected only in 15% of KP neurons. These observations raise intriguing questions about the physiological roles of these neuropeptides potentially co-released in the vicinity of GnRH neurons. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Homeostatic regulatory mechanisms Citation: Vida B, Deli L, Kalamatianos T, Hrabovszky E, Caraty A, Clive CW, Liposits Z and Kalló I (2009). Co-localisation of neuropeptides with kisspeptin in preoptic/hypothalamic neurons of the mouse brain. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.025 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Feb 2009; Published Online: 26 Feb 2009. * Correspondence: Imre Kalló, Hungarian Academy of Sciences, Budapest, Hungary, kallo@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Barbara Vida Levente Deli Theodosis Kalamatianos Erik Hrabovszky Alain Caraty Coen W Clive Zsolt Liposits Imre Kalló Google Barbara Vida Levente Deli Theodosis Kalamatianos Erik Hrabovszky Alain Caraty Coen W Clive Zsolt Liposits Imre Kalló Google Scholar Barbara Vida Levente Deli Theodosis Kalamatianos Erik Hrabovszky Alain Caraty Coen W Clive Zsolt Liposits Imre Kalló PubMed Barbara Vida Levente Deli Theodosis Kalamatianos Erik Hrabovszky Alain Caraty Coen W Clive Zsolt Liposits Imre Kalló Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.