COLLOIDAL SILVER NANOPARTICLES PREPARATION USING VARIOUS TYPES OF REDUCING AGENT AS ANTIBACTERIAL MATERIAL

Abstract

Silver nanoparticles are known as a good antibacterial activity and can be utilized in daily life. There are severalmethods for synthesis of silver nanoparticles, and one of the most widely used is by using chemical reduction. In this study colloidal silver nanoparticles were synthesized by using a variation of precursor concentration of AgNO 3 250, 500, and 750 ppm, as well as variation types of reducing agent which are ascorbic acid, citric acid, and trisodium citrate with Polyvinyl Alcohol as stabilizer. The antibacterial test were conducted by the disc diffusion method. The result of this study showed that the sample with a 250 ppm of precursor AgNO 3 with citric acid as reducing agents produce the optimum antibacterial activity with the same inhibition area to Staphylococcus aureus and Eschericia coli. The inhibition diameter is equal to the standard solution which is 8 mm at the same concentration. Analytical results obtained from the spectrophotometer gave maximum wavelength (λ max ) at 421 nm. Based on the analysis of particle size, it is obtained that the average particle size is 55 nm with a refractive index of 1.3328, the viscosity of colloidal silver nanoparticle 0.8878 and the polydispersity index of 0.3. Keywords: Silver Nanoparticle, Reduction, Antibacteria.