Abstract
Adding microbial transglutaminase (MTGase) to frozen surimi to enable the surimi to be sold as a higher-grade product at a higher price defrauds surimi product manufacturers and undercuts legitimate industry prices. Therefore, it is important to develop an accurate method of detecting the presence of MTGase in surimi. In this study, an immunochromatographic strip assay with a colloidal gold antibody probe was successfully developed and used to rapidly and qualitatively detect MTGase in surimi samples. The results were obtained in less than 10 min. The limit for the qualitative detection of MTGase using the immunochromatographic strip assay was identified as 1.0 μg/mL. The results of the immunochromatographic strip analysis of frozen surimi samples were verified by comparison with the results of a sandwich enzyme-linked immunosorbent assay. The colloidal gold probe-based immunochromatographic strip assay was thus found to be a rapid, economical, and user friendly method of detecting MTGase in surimi.
Highlights
Transglutaminase catalyses the intra- and/or intermolecular crosslinking of proteins by ε-(γ-glutamyl) lysine side-chain bridge [1]
Electrostatic charge adsorption was the primary method used to label the antibodies with the colloidal gold nanoparticles
A simple and rapid method of detecting microbial transglutaminase (MTGase) in surimi samples using an immunochromatographic strip assay with colloidal gold nanoparticles as labels was developed
Summary
Transglutaminase (glutaminyl-peptide:amine γ-glutamyltransferase, EC 2.3.2.13; TGase) catalyses the intra- and/or intermolecular crosslinking of proteins by ε-(γ-glutamyl) lysine side-chain bridge [1]. The enzyme has unique effects on protein properties, gelation capability, thermal stability, water holding capacity, and so forth [2,3,4]. It plays an essential role in a wide range of organisms and has been found in various animal tissues, fish, plants, and microorganisms [1, 5,6,7]. Some manufacturers use MTGase as a texture modifier to improve the gelation properties of surimi, enabling it to be sold at a higher price. MTGase rarely presents a health hazard, its addition to surimi defrauds surimi product manufacturers and undercuts legitimate industry prices. It is important to develop an accurate method of detecting MTGase in surimi
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