Collision cross sections of high-mannose N-glycans in commonly observed adduct states--identification of gas-phase conformers unique to [M-H](-) ions.

W B Struwe,J L Benesch,D J Harvey,K Pagel

doi:10.1039/c5an01092f

Collision cross sections of high-mannose N-glycans in commonly observed adduct states--identification of gas-phase conformers unique to [M-H](-) ions.

W B Struwe, J L Benesch + Show 2 more

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https://doi.org/10.1039/c5an01092f

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Journal: The Analyst	Publication Date: Jan 1, 2015
Citations: 38	License type: cc-by

Affiliation: St George's, University of London, University of Oxford, University of Greifswald, Fritz Haber Institute of the Max Planck Society

#Drift Tube Ion Mobility-mass Spectrometry #Ion Mobility-mass Spectrometry + Show 8 more

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Abstract

We report collision cross sections (CCS) of high-mannose N-glycans as [M + Na](+), [M + K](+), [M + H](+), [M + Cl](-), [M + H2PO4](-) and [M - H](-) ions, measured by drift tube (DT) ion mobility-mass spectrometry (IM-MS) in helium and nitrogen gases. Further analysis using traveling wave (TW) IM-MS reveal the existence of distinct conformers exclusive to [M - H](-) ions.

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