Collibacillus ludicampi gen. nov., sp. nov., a new soil bacterium of the family Alicyclobacillaceae.

Abstract

A novel moderately thermophilic aerobic bacterium, strain TP075T, was isolated from soil collected from an athletic field in Japan. Strain TP075T is a rod-shaped, aerobic bacterium that forms terminal endospores. The KOH lysis test suggested that the cell wall of the isolate has a Gram-positive structure. For aerobic growth, the optimum pH and temperature were 4.0–5.0 and 47–50 °C, respectively. Draft genome sequencing showed that the G+C content of genomic DNA was 46.5 mol%. Branched-chain fatty acids (iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0) were the major components of the cellular fatty acid profile. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain TP075T belongs to the family Alicyclobacillaceae , with the highest similarity to Effusibacillus consociatus CCUG53762T (92.6%) and Tumebacillus soil CAU11108T (92.5%). Genome-based analyses indicated that strain TP075T and the most closely related strain, Effusibacillus pohliae DSM 22757T, share an average amino acid identity value of 62.57% and an average nucleotide identity value of 70.86 %. The results obtained in this study suggest that strain TP075T represents a novel species of a novel genus, for which we propose the name Collibacillus ludicampi gen. nov., sp. nov. with type strain TP075T (= JCM 34430T=TBRC 15189T).