Abstract
A total of 24 Colletotrichum isolates were isolated from diseased Japanese plum (Prunus salicina) fruits showing chlorotic regions with whitish-brown sunken necrotic lesions and phylogenetic relationships among the collected Colletotrichum isolates were determined. A subset of 11 isolates was chosen for further taxonomic study based on morphology and molecular characteristics identified using the internal transcribed spacer (ITS) and beta-tubulin (TUB2) genes. Isolates in the C. acutatum complex were analyzed using partial sequencing of five gene regions (ITS, GAPDH, ACT, TUB2, and CHS), and C. gloeosporioides sensu lato (s.l.) isolates were analyzed using seven gene regions (ITS, TUB2, GAPDH, ACT, CAL, CHS-1, and ApMat). Morphological assessments in combination with phylogenetic analysis delineated four species of Colletotrichum including C. gloeosporioides sensu stricto (s.s.), C. nymphaeae, C. foriniae, and C. siamense; these data identify Colletotrichum fioriniae and C. siamense two new species associated with plum anthracnose in South Korea. Finally, the pathogenicity of these four species in the development of plum anthracnose in South Korea was confirmed by inoculations of plum fruit.
Highlights
Japanese plums (Prunus salicina Lindl.) are delicious stone fruits, which have a wide variety of uses
A total of 24 Colletotrichum isolates were isolated from Japanese plum fruits collected from different commercial orchards exhibiting anthracnose in Sangju, South Korea (Gyeongbuk Province)
Colletotrichum isolates belonging to C. gloeosporioides s.l., (12 isolates) and C. acutatum s.l., (12 isolate) were first delineated using the combined internal transcribed spacer (ITS) and TUB2 align sequence data set for phylogenetic analysis (Fig. 1)
Summary
Japanese plums (Prunus salicina Lindl.) are delicious stone fruits, which have a wide variety of uses. Anthracnose of Japanese plum caused by Colletotrichum species has been reported in Korea[3,9]. Colletotrichum species have been reported to caused anthracnose in common fruits in Korea, such as apples, grapes, peaches, and persimmons[12,13,14,15]. The use of a five genes phylogenetic analyses along with morphological characters to identify C. nymphaeae as the causative agent of plum anthracnose revealed that Colletotrichum species associated with plum anthracnose in Korea may have a remarkable species diversity[9]. This study sought to investigate species diversity within Colletotrichum isolates related to plum anthracnose in Sangju, Korea based on combined morphological and multigene phylogenetic strategies, followed by a pathogenesis analysis of the different identified Colletotrichum species on plum fruit
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
