Colletotrichum kahawae subsp. cigarro causing leaf spot disease on arecanut, Areca catechu L. in India: A first report

Abstract

Arecanut is a prominent cash crop in India, known for its potential to significantly boost per capita income. The presence of fungal diseases poses a significant constraint to arecanut cultivation. In October 2019, a severe outbreak of leaf spot disease with 90°% disease incidence was recorded in the farmer's arecanut gardens from Kalasa hobli, Chikkamangaluru, Karnataka, India. Leaf spot symptoms started as small, irregular, light-dark brown spots (3–10 mm in diameter) with a yellow halo on the leaf lamina, which later coalesced to form larger necrotic lesions and a blighted appearance. The symptoms were also observed on leaf sheaths and nuts. In the present study, we identified and characterized the pathogen causing leaf spot disease using cultural, morphological, molecular characterization using multi-gene phylogeny, and pathogenicity assays. Microscopic, and molecular identification of CPCRICkc7G isolate retrieved from the leaf spot infected sample established the association of Colletotrichum kahawae subsp. cigarro as the causal agent. Pathogenicity assays confirmed the Koch's postulate by reproducing the field observed symptoms. To best of our knowledge, this confirms the first report of C. kahawae subsp. cigarro causing leaf spot disease on arecanut in India. The findings from this current study play a pivotal role in enhancing the knowledge of arecanut diseases in India, and lay an essential foundation for devising effective disease management approaches to curtail the disease spread.