Collection time, dehidration, culture media and environment for germination and storage of campomanesia guazumifolia (cambess.) O. Berg. Pollen

Karina Guollo,Juliana Cristina Radaelli,Américo Wagner Junior,Welida Mayara Tomazoni Keller,Carlos Kosera Neto

doi:10.1590/0100-29452021996

Karina Guollo, Juliana Cristina Radaelli + Show 3 more

Open Access

https://doi.org/10.1590/0100-29452021996

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Abstract

Abstract Sete-capote tree, a neglected species, has great ecological, cultural and potential economic importance, however, studies for this and other native species of the Myrtaceae family are still scarce. The objective of this study was to elucidate aspects of pollen germination and viability of this specie. For the experiment, pollen from flowers in pre and post anthesis was used, which was dehydrated in a chamber containing silica, for different periods. For germination tests, different concentrations of sucrose, boric acid and calcium nitrate were used. After obtaining germination results above 80%, the pollen grains were stored in refrigerator (5 °C), freezer (-17 °C), liquid nitrogen (-147 °C) and natural environment (± 25 °C), evaluating monthly the germination, until total loss of viability. For germination, it was recommend using pollen from flowers in post-anthesis, dehydrated for 24 hours in a silica chamber. The culture medium should contain 12% sucrose (C12H22O11), 10% boric acid (H3BO3) and 20% calcium nitrate (Ca(NO3)2) to obtain high germinative percentages. In addition, pollen presents orthodox behavior and when stored in liquid nitrogen, remains viable for 30 days.

Highlights

Material and methodsThe species Campomanesia guazumifolia (Cambess.) native to the Brazilian flora, popularly known as seven-capote tree, belongs to the Myrtaceae family and occurs commonly in the States of Rio de Janeiro, Minas Gerais, São Paulo, Mato Grosso do Sul even Rio Grande do Sul, in almost all groves and forests
The fruits have the potential to be commercialized, both in natura, as well as juices, jellies, ice cream and other sweets, studies that aim at the commercial management of the species to reach a wide scale of commercialization are still scarce. Those that have already been carried out basically comprise the morphological characterization of flowers and fruits (DONADIO; MORO; CUNHA, 2017; LIMA; GOLDENBERG; SOBRAL, 2011; SOUZA; SOUZA; PANOBIANCO, 2018), physicochemical, phytochemical characterization and biological and antimicrobial activities (ARRUDA, 2013; BERNARDO et al, 2015; GOLDONI, 2017), in addition of seed germination (GOLDONI, 2017; SOUZA et al, 2018), and characterization of its essential oil (STEFANELLO et al, 2011)
The germination of pollen grains obtained from post-anthesis flowers was approximately 35%, benefiting from dehydration for 24 hours (Figure 1)

Summary

Introduction

Material and methodsThe species Campomanesia guazumifolia (Cambess.) native to the Brazilian flora, popularly known as seven-capote tree, belongs to the Myrtaceae family and occurs commonly in the States of Rio de Janeiro, Minas Gerais, São Paulo, Mato Grosso do Sul even Rio Grande do Sul, in almost all groves and forests. Given the above and the scarcity of research on native species of the Myrtaceae family, the objective of this study was to elucidate aspects of pollen germination and viability of sete-capote tree. Experiment 2 After obtaining the results of experiment 1, the concentration of sucrose in the culture medium was evaluate by testing five levels (0, 10, 20, 30 and 40%), using the best conditions in experiment 1.

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