Abstract
Peripheral blood stem cells (PBSC) were collected from six children weighing less than 17 kg. Stem cell pools had been expanded by chemotherapy and rhGM-CSF. Nineteen procedures were performed with a continuous-flow separator (CS 3000 plus) using central venous access. The extracorporeal line was primed with red blood cells and 5% albumin (HA). Acid-citrate-dextrose:-formulae A(ACD-A) was added in a median ratio 1:11 (range 1:9–1:15). A median number of 5.6 × 10 8kg −1 NC (3.5–9.7) and 7.1×10 6kg −1CD34+ cells (2.1–26.1) were collected per patient. Four patients were transplanted with PBSC and showed normal haematopoietic recovery (leucocytes >1000 μL −1 between days 11 and 12). The results show that successful PBSC collection and transplantation is possible even in small paediatric patients. Administration of growth factors results in a marked increase of peripheral white blood cells and in a higher yield of NC and CD34+ cells in PBSC collections.
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