Collection of capillary electrophoresis fractions on a moving membrane.

Abstract

AbstractDuring the last 10 years, capillary electrophoresis (CE) has developed into a powerful analytical method for a wide variety of applications. It tends to replace traditional slab-gel electrophoresis for the separation of complex biological samples (DNA, proteins, carbohydrates) (1). The ability to further analyze the separated components using specific secondary reactions, analogous to well-established methods such as the Southern blot (2) and direct blotting (3) is important for the full development of this rapidly growing method. The total amount of sample separated in CE is very small when compared to slab gels, but thanks to the sensitivity and miniaturization of modern detection methods, it may still be sufficient for many postseparation analysis applications, such as hybridization, sequencing, enzyme digestion, reamplification by polymerase chain reaction (PCR), and so on.KeywordsCapillary ElectrophoresisSeparation MediumCollection DeviceHydroxypropyl CelluloseFraction CollectionThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.