Collection, Identification, Phytochemical analysis and Phyto toxicity test of Wood inhabiting Fungi Ganoderma lucidum (Curt.Fr.)P.Karst.

Abstract

Plan: In this study 10 strains of mushroom Ganoderma lucidum were collected from various places and decided to screen their phytotoxicity activity and phytochemical constitution. Preface: The popular mushroom Ganoderma lucidum (Reishi) was a bracket fungus and has been widely used for the promotion of health and longevity in Asian countries. The dried powder of G.lucidum was popular as a cancer chemotherapy agent in ancient China. G.lucidum clearly demonstrates anticancer activity in experiments with cancer cells and has possible therapeutic potential as a dietary supplement or alternative therapy for breast and prostate cancer. And also the fruiting bodies of Ganoderma lucidum (Polyporaceae) are a well known Chinese crude drug identified as a phytotoxic agent. H owever, since G.lucidum was available from different sources, it is advisable to test its biological activity. Methodology: G.lucidum strains collected was isolated and identified with potato dextrose agar medium. Then the secondary metabolites were qualitatively studied through phytochemical analysis. Outcome: Significant root length inhibition was observed at 100ppm and 200ppm. Similarly seed germination was also significantly inhibited at the concentration 100ppm and 200ppm extracts. The mean data of root length inhibition by ethanol extract in 100ppm was 0.393 and 0.208 in 200ppm. Likewise the mean data of seed germination inhibition by ethanol extract in 100ppm was 15 and 0.208 in 200ppm. Overall results supported that Ganoderma lucidum clearly suppo rted the wider medicinal uses and established its anticancer activity.