Collateralized projections from neurons in the rostral medulla to the nucleus locus coeruleus, the nucleus of the solitary tract and the periaqueductal gray

Abstract

We have examined collateral projections of locus coeruleus afferent neurons in the rostral medulla to the caudal nucleus of the solitary tract or to the periaqueductal gray using double retrograde labeling techniques in the rat. The present findings confirm previously reported connections to the locus coeruleus, the nucleus of the solitary tract and the lateral periaqueductal gray from the nucleus paragigantocellularis in the rostral ventral medulla. Our results also reveal previously unreported projections from the rostral dorsomedial medulla (in a similar region as locus coeruleus-projecting neurons) to the lateral periaqueductal gray. Following retrograde tracer injections into the nucleus of the solitary tract and the locus coeruleus, doubly labeled neurons were seen in both the nucleus paragigantocellularis and in the rostral dorsomedial medulla. Cell counts revealed that approximately 25% of locus coeruleus-projecting neurons in the nucleus paragigantocellularis, and 12% in the dorsomedial medulla, also innervate the caudal nucleus of the solitary tract. In contrast, no doubly labeled neurons within the rostral ventral medulla were found following injections into the lateral periaqueductal gray and the locus coeruleus, although singly labeled neurons for the two tracers were interdigitated in some regions. Following these injections, numerous neurons were also retrogradely labeled in the dorsomedial medulla in the region of the medial prepositus hypoglossi and the perifascicular reticular formation. A small percentage of locus coeruleus afferents in the dorsal medulla (approximately 10%) also projected to the lateral periaqueductal gray. These results indicate that neurons in both the ventrolateral and dorsomedial rostral medulla frequently send collaterals to both the locus coeruleus and the caudal nucleus of the solitary tract. A small number of neurons in the dorsomedial medulla project to both the locus coeruleus and the lateral periaqueductal gray, but separate populations of neurons project to the locus coeruleus and the lateral periaqueductal gray from the ventrolateral medulla. These results functionally link the locus coeruleus and the nucleus of the solitary tract by virtue of common afferents, and support other studies indicating the importance of central autonomic circuitry in the afferent control of locus coeruleus neurons.