Collateral Prospects of Polyherbal Formulation as Antidiabetic and Appraising its Toxicity in Early Development of Zebrafish (Danio Rerio) Larvae

Avila Jerley Arockiya,Lakshmi Pavithra,Anita Margret Arockiya

doi:10.47743/jemb-2021-55

Abstract

The polyherbal formulation was prepared using leaves, flowers, fruits of these specific plants  Ficus religiosa, Allium sativum, Senna auriculata, Andrographis paniculata, Momordica charantia, Eugenia jambolana (FASAME). The hydroalcoholic extracts of the FASAME and its phytochemical constituents was determined. For toxicity study, the zebra fishes are used as the experimental models and were exposed to different concentration of FASAME formulation (100 μg, 70 μg, 50 μg, 10 μg, and 5 μg). Then its viability and its lethal effect was calculated. Finally the antidiabetic activity of FASAME was determined by β - galactosidase assay.The phytochemical constituents like flavonoids, steroids, terpenoid, saponins, resins, carbohydrates, proteins and essential oil was present in the hydroalcoholic extract of FASAME polyherbal formulation .The effect of FASAME formulation on Zebra fish larvae has a substantial increase in the percentage of viability at their lower concentration. The FASAME formulation exhibited selective anti-diabetic activity. These results showed that the FASAME formulation has anti-diabetic activity and can be used for the treatment of diabetics.The phytochemical analysis of FASAME drug has a mixture of phytochemical compounds and their IC50 value was found to be 74.39 μg/ml. The toxicity analysis on Zebra fish larvae also showed higher viability at their lower concentration and proved to be a powerful antidiabetic drug for diabetics.

Highlights

Diabetes mellitus is a chronic disease that last long with several disorder associated both metabolically and physically.[1]
In this work we focused to develop a polyherbal formulation for the treatment of diabetes
We tested the antidiabetic effect of FASAME, a polyherbal formulation by β-galactosidase assay and their toxicity using zebra fish model

Summary

Introduction

Diabetes mellitus is a chronic disease that last long with several disorder associated both metabolically and physically.[1]. The successful natural approach of treating these diseases has paved the new way in drug discovery.[2] there are many drugs which are consumed orally or injected intravenously to control the blood sugar level, they may develop some side effects and are of high cost This condition leads to exploration of the traditional herbal plants which can have complete cure without any side effects. In India it was prevailed thousands of years ago, and it is acknowledged as the “Mother of All Healing”.3 In this system of medicine, nearly 600 different plants have capacity to overcome diabetes and it is scientifically reported in some of the Indian books like Charak, Samhita, Mahdhav Nidan and Astang Sanghra.[4] There are more than 12,000 plants that have shown good have ethnopharmacological effect against diabetes, but most of them lack in scientific authentication.5Recently certain therapies are using oral hypoglycaemic tablets which does not restores the normal glucose level and the have side effects.[6] This is considered as an immense challenge which needs to resolved by exploring the efficiency of herbs which divulge conventional antidiabetic activity. Further it exhibits a greater genomic and physiological resemblances to humans .Drug monitoring and accessing its

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