Abstract
Collateral projections of single neurons in the midbrain periaqueductal gray (PAG) to the nucleus accumbens (ACB) and nucleus raphe magnus (NRM) were observed in the rat by a fluorescent retrograde double-labelling technique. After injecting propidium iodide into the ACB and bisbenzimide into the NRM, doubly labelled PAG neurons were most frequently seen in the ventrolateral subnucleus and ventral part of the medial subnucleus at the middle and caudal levels of the PAG.
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