Abstract

Abstract Cell-based therapies such as Scaffold, stem cells, and secretome, are one of the alternatives to enhance the regeneration of hyaline-like cartilage in cases of cartilage defects. This study is an in-vivo experiment using animal models, in which we apply a composite of DFLP (Dwikora-Ferdiansyah-Lesmono-Purwati) Scaffold and Adipose-Derived Stem Cells (ASCs) or Secretome to an injury model on the distal femoral trochlea of New Zealand White Rabbits. The animals were divided into four groups: (1) control (K); (2) Scaffold only (S); (3) Scaffold + ASCs (SA); (4) Scaffold + Secretome (SS). Animals were terminated in the 12th week, and an immunohistochemistry (IHC) evaluation for Collagen type I and II were done. Statistical analysis shows that collagen type I IHC between groups shows no significant difference (p = 0.546). Collagen type II IHC shows significant difference between groups (p = 0,016). The findings in this study showed that Scaffold + ASCs group and Scaffold + Secretome have better collagen type II expression compared to the control group. DFLP Scaffold composite with ASCs or Secretome shows potential for cartilage regeneration therapy by increasing type II collagen expression as in hyaline-like cartilage which may be used for regenerative therapy for cartilage defects. Keywords : DFLP Scaffold; Adipose-Derived Stem Cells (ASCs); Secretome; Collagen Type I; Collagen Type IICorrespondence : ianperbowo@me.com

Highlights

  • 5) Stem Cell Research and Development Center, UTnraivuermsitaatsiAcirlsaengvgear,eSurbarbaayina, Inindojunersyia is a fatal injury, with a Submitted : Januari 2019 mortality rate of up to 50%

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Summary

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5) Stem Cell Research and Development Center, UTnraivuermsitaatsiAcirlsaengvgear,eSurbarbaayina, Inindojunersyia is a fatal injury, with a Submitted : Januari 2019 mortality rate of up to 50%. Accepted : February 2019 experience severe brain injury in the United States. SKuebymwoitrtdesd: : February 2020 Coeclclu-brsasiendthethfeirsatp2iews eseukcshaftaesr thSecainffjoulrdy,. PThSecraefffoorled, cionmthpeosciatsee wofitbheiAngSChasnodrledSeimcrpertoompeerlys,hiotwcasnpbortienngtidaelatfho.r cartilage regeneration therapy by increasing type II collagen. ABSTRAK expression as in hyaline-like cartilage which may be used for Cedera otak berat traummaattiiss aaddaallaahh cceerdedegereraaneffaraattaatillv, edetnhngegraannptytiinnfgogkrkaacttakrkteeimlmagaatetiiaadnnefhheicinntgsgg.gaa5500%%. Sekitar 1,5 jjuuta orang mengalami cedera otak berat di Amerika Serikat. ADDniieaasbtbheeetsetiseaiswnaisnispdsiodipnueisdininuitispalailntyiebpnysapwtreiimetnhedstircwaatuiiomtnhattircasuevmeraetbircasinevinejruerybrain injury ukYYsg1uuin)dddgRhihaae0azs.eA2iApddaedmimnigPt,P/orkraefgaabAcboanhtowrewosowpt1ohai,ne1sPse,irgPosailurvonlaegafnnayntadeainnaatddrnSaadIumnSrt1uyeu.sna0rcsAyuimvlaiaergrAl/,Cainarrgleagnao2fgRgaS2UD Dr Soetomo, Medical Faculty of co1n)tiRAneuirselidadnegbngyt aofkUeAntanivmeesirtnsheietysi2.o0logmyga/kngd, InatnednstihveenCare of RSUD Dr Soetomo, Medical Faculty of m2a)inAStatiairnlfafendDgeugpsaainUrtgenmiavdeednristtiiotoyfn.Aalne1s0thmesgio/kloggdyoasnedifReanimation of RSUD Dr Soetomo, Medical Facth2e)reSuwtlatyaffsoDaf erAepaaircrlttaeionmngegfnartoUomfnAtihvneerrssaittbhybe.sitiso.logy and Reanimation of RSUD Dr Soetomo, Medical Faculty of Airlangga University

ASCs Preparation
Statistical Analysis StatisWticealdiAdnaaslytastiisstical analysis using
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