Abstract
THE proline analogue, cis-hydroxyproline, when incorporated into collagen, prevents collagen secretion1 and also inhibits the growth of fibroblasts in vitro2. We describe here studies on the mechanism of action of cis-hydroxyproline on cell proliferation of primary cultures of murine connective tissue cells and of their spontaneously transformed, tumorigenic counterparts. We find that the analogue inhibits the attachment, spreading and proliferation of the connective tissue cells and that these effects are completely reversed when the cells are grown on collagen-coated dishes. The tumorigenic cells are less affected by cis-hydroxyproline even though their collagen synthesis is inhibited to a similar extent to the connective tissue cells. These results suggest that the presence of a collagen matrix is required for the growth of some connective tissue cells in vitro but not for the growth of their tumorigenic counterpart.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.