Abstract
Measurement of collagen turnover is complicated by the reutilization of isotopicprecursors used to label the collagen. In an earlier study a novel approach was used to circumvent the problems of precursor recycling and unusually short half-lives were determined for collagen in adult rat periodontal tissues (Sodek, 1977). To verify these results we have used an alternate procedure devised by Poole (1971) in which the decay profile for the radiolabelled protein is corrected in accordance with the decay of the radiolabelled precursor. In this manner real half-lives for mature, neutral salt-insoluble collagen were determined as 3 days in the molar periodontal ligament, 6 days in the continuously erupting incisor ligament and approximately 10 days in the lamina propria of the gingiva, compared to apparent half-lives for these tissues of 6, 12 and approximately 20 days, respectively. The values calculated for actual half-lives are, therefore, approximately two-fold faster than values determined without compensating for reutilization, a difference that is in agreement with other protein turnover studies in which the effects of precursor reutilization have been measured. Although the real half-lives determined in this study indicate turnover rates for the periodontal tissues that are slightly slower than reported previously, the relative differences between the tissues in the rates of collagen turnover are similar. Moreover, the study confirms the existence of a remarkably high rate of collagen remodelling in these tissues.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have