Abstract
Cloned colonies and explants of embryonic chick retinal pigmented epithelium from donors of various embryonic ages were maintained in culture for different periods and examined by electron microscopy. The cells appeared morphologically differentiated and polarized. Basement-membrane material and striated collagen fibrils were identified as extracellular deposits beneath the basal surfaces of the cells. There appeared to be a distinct spatial and temporal correlation between the production of basement-membrane material and collagen fibrils. Increasing donor age correlated positively with increasing average diameter of the collagenous fibrils produced, as well as a widening of the range of fibril sizes.
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