Collagen-Niflumic Acid Spongious Matrices for Bone Repairing

Abstract

Collagen is one of the most used biomaterials for bone defects repair, proving good results in tissue reconstruction research, and also its features recommend it as a very attractive drug delivery scaffold for local treatment of the affected osseous tissue. The inflammatory response is a common reaction that occurs in bone disease, the topical administration of anti-inflammatory drugs (NSAIDs) representing a reliable strategy to overcome this issue. The purpose of this paper was the physical-chemical and biopharmaceutical evaluation of some spongious matrices consisting of collagen as release support and niflumic acid as drug NSAID model, usable in bone tissue regeneration. Type I fibrillar collagen gel (2.4% w/w, 3.5 pH) was extracted from calf hide by the technology currently used in Collagen Department of Division Leather and Footwear Research Institute. The collagen sponges were obtained by freeze-drying of gels adjusted at 1% and 7.3 pH, with different dextran (0; 10 and 20%) and MgO (0; 30 and 60%) concentrations (reported to dry collagen), with 0.5% and without niflumic acid (NA) (reported to gel) and the same amount of glutaraldehyde (0.5% reported to collagen dry substance). The sponges were evaluated through water absorption, FT-IR spectroscopy and optical microscopy. In vitro NA release from the designed sponges was carried out using a sandwich device adapted to a dissolution equipment. Power law kinetic model was applied to explain drug release from the tested formulations. The NA release from collagen sponges showed a non-Fickian transport mechanism. The addition in different concentrations of dextran and MgO leads to more compact structures and improves stability of collagenic matrices. Our results showed that the designed support could be adequate for treating the inflammation associated with a bone defect in orthopedic surgery.