Abstract
A technique for studying small granulating wounds in man and laboratory animals is described. The wounds are produced and later excised using standard skin punches. Using this tissue we have determined the profile of a number of biochemical steps in collagen biosynthesis occurring in the early stages of wound healing. The most useful indicator of collagen synthesis was measurement of protocollagen proline hydroxylase (PPH) activity the enzyme which hydroxylates peptide-bound proline. In rat skin PPH-activity reaches a maximum 5 days after wounding. During starvation, the level of PPH-activity falls within a few days as does the soluble collagen content indicating decreased collagen synthesis. Systemic cortisone administration decreases the PPH level in wounds, and the effect can be reversed by adding systemic vitamin A (which has no effect by itself). Preliminary studies in humans indicate that similar elevations in PPH-activity occur in 5-day-old wounds. Patients with scleroderma have higher baseline levels of PPH-activity in their skin but show a normal response of PPH-activity to wounding.
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