Collagen hydrolysates from deer tendon: Preparation assisted with different ultrasound pretreatment times and promotion in MC3T3-E1 cell proliferation and antioxidant activities

Abstract

In this study, the hydrolysates of deer tendons with antioxidant activity and promoting bone proliferation activity were prepared using ultrasound. The results indicated that ultrasound (60 min) treatment had noticeable effects on the structure and enzymolysis degree of collagen protein, including looser structure, less α-helix/β-turn, more hydrophobic groups, and a higher degree of hydrolysis in comparison with others. Besides, compared with unpretreated collagen hydrolysates (CPHs), the hydrolysates with ultrasound treatment (60 min) showed significant effects on bone proliferation (cell proliferation rate: 63.9 ± 4.8%), alkaline phosphatase activity (14.488 U/mg), and antioxidant activities (DPPH radical scavenging rate: IC50 =4.05 mg/mL and ferric ion reducing antioxidant power (FRAP): 0.17 mmol Fe2+/mg). CPHs (ultrasound 60 min) also could protect MC3T3-E1 cells from H2O2-induced damage by reducing the ROS and [Ca2+]i. Taken together, these results might provide a basis for the ultrasound-assisted preparation of potential antioxidant hydrolysates to be applied in the food industry.