Collagen Hydrolysate against Fluvoxamine Maleate-Induced Osteoporosis in Albino Rats: A Histological and Immunohistochemical Study

Abstract

Background: Osteoporosis (OP) is a prevalent degenerative bone disease among patients receiving selective serotonin reuptake inhibitors as fluvoxamine maleate (FM). Collagen hydrolysate (CH) is a nutritional component that has antiresorptive effect.Aim of Work: Evaluate the possible protective effect of CH against FM-induced OP in adult male albino rats.Material and Methods: Thirty six rats were divided into 4 groups; group I (control), group II (OP group): injected with FM daily for 4 weeks, group III (CH group): received FM concomitant with oral CH for 4 weeks, group IV (recovery group]: received only FM for 4 weeks & were left without taking any drugs for another 4 weeks. Total serum alkaline phosphatase (ALP) and calcium (ca+2) were measured. Bone specimens from the right femurs and first lumbar vertebrae were processed for HE osteocytes with pyknotic nuclei inside widened lacuna, widened haversian canals. Bone matrix showed fain areas, cavitations & multiple resorption cavities with osteoclasts. There was significant reduction in the mean thickness of compact bone, the mean area of trabecular bone, area % of OPN & mean number of PCNA +ve cells compared to control. Group III exhibited significant reduction in ALP & elevation in Ca+2. The bone showed preserved histological architecture almost as the control. There was significant increase in the mean thickness of compact bone, the mean area of trabecular bone, area % of OPN & mean number of PCNA +ve cells compared to groups II & IV.Conclusion: CH has a potential osteoprotective effect against FM-induced osteoporosis